Digital Image Processing

I am trying to develop a image processsing application that will analyse some grayscale images, the images in question are basically biological photographs(PCR GEL Photographs) that have some bands which are lighter in shades, and these bands are arranged in some pattern( Mostly linear), Till now, i had suceeded to programatically identify those bands which are distinct and clearly seperated from other band, but the band, which are close, could not be identified as seperate band.
more over my algorithms fails miserably in case case of color photographs,and i am clueless for a better approach.

i will be grateful if somebody out there had any previous experience,to help me out
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