cannot run the subfolder in the server,,please help me

Im a new bee to I have encountered errors while running the subfolder("used as test folder")

Configuration Error
Description: An error occurred during the processing of a configuration file required to service this request. Please review the specific error details below and modify your configuration file appropriately.

Parser Error Message: It is an error to use a section registered as allowDefinition='MachineToApplication' beyond application level. This error can be caused by a virtual directory not being configured as an application in IIS.

Source Error:

Line 24: ASP.NET to identify an incoming user.
Line 25: -->
[color=Red]Line 26: [/color]
Line 27:
Line 28: <!--
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